아래와 같이 PCBMI Seminar를 개최하오니 관심있는 연구자 및 T cell, AUTOIMMUNITY, TB 연구 관계자분들의 많은 참여 바랍니다.
- 일 시: 2009년 12월 11일(금) 오전 10:30~12:00
- 장 소: 포-가 연구원 1205호 세미나실 (가톨릭대 성의교정 성의회관 12층)
- 발표연자: Paul V. Lehmann, M.D., Ph.D., Professor of Pathology at C.W.R.U, Founder, President and CEO of CTL
- 발표제목: ELISPOT assays for delineation of T cell effector classes and activation states
- 초 록
During this talk I will cover our studies utilizing granzyme B, perforin, TRAIL and IFN-g ELISPOT assays for monitoring HIV-specific T cells in infected individuals, detecting antigen-specific effector, memory, and “unhelpted/frustrated/lethargic” CD8 cells. The data draw attention to the need for multicolor ELISPOT analysis which permits to combine the strengths of the ELISPOT approach (ultra high sensitivity, robustness, need for minimal cell material, suitability for high throughput and regulated tests, and work with cryopreserved PBMC) with the need for multiplexing. I will also cover the progress we have made towards the development, optimization, and qualification of simultaneous measurements of IL-2, IL-4, IL-17 and IFN-g to assess Thpp, Th2, Th-17 and Th1 effector cell diversity within antigen-specific CD4 cell immunity. the progress we have made towards the development, optimization, and qualification of simultaneous measurements of IL-2, IL-4, IL-17 and IFN-g to assess Thpp, Th2, Th-17 and Th1 effector cell diversity within antigen-specific CD4 cell immunity.
조회 : 10,120
