1. 일     시: 2009년 5월 12일(화) 11:00~12:00

   2. 장     소: 포-가 연구원 1305호 회의실

   3. 발 표 자: 도윤경 박사 (울산과기대)

   4. 발표제목: Targeting of LcrV virulence protein from Yersinia pestis to DCIR2+ dendritic cells, protects mice against pneumonic plague

   5. 초     록:
A vaccine against pneumonic plague does not yet exist for humans, at a time when antibiotic-resistant bacteria are being identified and Yersinia pestis (Y. pestis) is a potential biological weapon. A subunit vaccine, comprised of the F1-V protein in alhydrogel, provides antibody-based protection in small animal models, but recent studies suggest a value for cellular immunity in addition.  To achieve this, we targeted the Y. pestis LcrV protein directly either to CD8α+DEC-205+ or CD8α-DCIR2+ dendritic cells (DCs) and used poly IC as a clinically feasible adjuvant.  The anti-DEC:LcrV vaccine induced polarized Th1 immunity including high frequencies of IFN-γ secreting CD4+ T cells, while the DCIR2+ DCs-targeted anti-DCIR2:LcrV vaccine induced fewer CD4+ T cells secreting IFN-γ, but higher IL-4, IL-10, IL-13, and IL-5. Surprisingly DCIR2+ targeting enhanced antibody titers and longevity. When vaccinated mice were challenged with virulent CO92 strain of Y. pestis, protection was closely correlated with anti-LcrV antibody levels, which were highest with anti-DCIR2:LcrV vaccine. These data indicate that anti-DCIR2:LcrV vaccine elicits broad and combined humoral and cellular immunity, as well as protection in a mouse model of pneumonic plague.

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